This study dealt with staff development and academic staff output at the universities in South-South geo-political zone of Nigeria. It was an Ex-post facto design study, carried out specifically in three federal universities in the area of study.These were: University of Port Harcourt, Choba; University of Uyo, Uyo and University of Calabar, Calabar.Two hypotheses based on the variables were formulated to guide and direct the study.These were: staff in-service training and staff ICT training, respectively. Two self-constructed questionnaires called staff development questionnaire (SDQ) and academic staff output questionnaire (ASOQ) were used for data collection. The first instrument was administered to 800 academic staff.The second instrument, three copies were administered one each to three students to assess each individual academic staff.Data collected were analyzed using one-way analysis of variance (ANOVA) and independent t-test. The results revealed among other things that; there existed an influence of in-service training on academic staff output, which is not significant. Statistically, there existed a difference between output of staff who received ICT training and that of those who had not; but the difference is not statistically significant.