Azoospermia, a condition characterized by the absence of sperm in a man's semen, affects approximately 1% of the
male population and accounts for about 10 to 15% of male infertility cases. This study aimed to assess the fertility
hormone levels in Azoospermia patients in Owerri. A cross-sectional study included twenty-five Azoospermia
patients attending the fertility clinic at Imo Specialist Hospital and twenty-five healthy individuals as a control
group. Venous blood samples were collected and analyzed for serum testosterone, follicle-stimulating hormone
(FSH), and luteinizing hormone (LH) using the ELISA method. The results indicated that infertile subjects had
significantly higher levels of FSH (9.91 ± 3.69 IU/L) compared to control subjects (5.54 ± 0.90 IU/L) (P = 0.0001).
Similarly, infertile subjects showed significantly higher levels of LH (8.50 ± 2.63 IU/L) compared to control subjects
(4.75 ± 1.15 IU/L) (P = 0.0001). In contrast, the testosterone levels in infertile subjects (2.56 ± 0.97 IU/L) were
significantly lower than those in control subjects (4.80 ± 1.19 IU/L) (P = 0.0001). In conclusion, the findings of this
study demonstrate a significant increase in gonadotropins (FSH and LH) and a significant decrease in testosterone
levels in males with Azoospermia.