Abstract

This study was conducted to characterize  E. coli  isolates in Coturnixcoturnix japonica (Japanese quails). A total sample of 80 cloacal swabs was collected  (40 from 2 months old quails and 40 from 3 weeks old quails). The isolates  were confirmed using  culture, microscopy  and biochemical identification. Molecular typing using Polymerase chain Reaction(PCR) assay confirmed  that 60 (75%)  of the isolates to be  E. coli and none of the isolates confirmed belonged  E. coli 0157 serotype. Statistical analysis using Chi square showed  no significant  difference in prevalence of E.coli between the 2 months old and the 3 weeks old quails (P>0.05). Antibiotic susceptibility testing showed that there was high resistance of  75%  of the E. coli isolates towards multiple antibiotics such as tetracycline and ampicillin while  sensitivity of 75% was found for ciprofloxacin and gentamycin. Our findings suggests that E. coli infection may be endemic in the study area but serotype  E. coli 0157  may be absent. The  multiple drug resistances dissipated in our study calls for concern in the face of increasing limited antibiotic options and more searh for genuine antibiotics in managing colibacillosis on Malaysian  quails. Proper hygiene  regime and biosecurity measures are necessary to minimize the risk of spread of  infection to the human population.