Abstract

This study was carried out to evaluate the expression of microsatellite protein (MLH1) on colorectal cancer specimen.
A total of 32 tissue specimens of colorectal carcinoma from Meena Histopathological and Pap smear Screening
Centre Jos and Federal staff Hospital Abuja were reviewed and analyzed Immunohistochemcially for the expressionof
MLH1 and MSH2 protein with colorectal cancer samples. Nineteen (59.3%) of colorectal cancer revealed negative
reaction to MLH1 protein and 17(53.1%) showed negative reaction to MSH2 protein, while 14(43.7%) showed
negative reaction to MLH1 and MSH2 protein (mismatch repair protein negative) indicating absence of DNA
mismatch repair protein. In 10 (31.2%) were positive reaction to MLH1protein (mismatch repair protein positive).
Fourteen (43.7%) showed moderate differentiated adenocarcinoma with haematoxylin and eosin technique while in
10(31.2%) were observed well differentiated adenocarcinoma as 8(25%) revealed poor differentiated adenocarcinoma
with the same technique. Four (28.5%) tissue specimens of moderate differentiated colorectal cancer showed stage II
of colorectal cancer with MLH1 protein and 1(7.1%) revealed stage III of colorectal cancer with MLH1 protein. In
3(30%) of well differentiated colorectal cancer were observed stage II of colorectal cancer with MLH1 protein and
2(20%) of same showed stage IV of colorectal cancer with MLH1 protein. Two (25%) poor differentiated
adenocarcinoma were stage II of colorectal cancer with MLH1 protein and 1(12.5%) showed stage IV of colorectal
cancer with MLH1 protein.