KIU Publications
Publications Archive
Explore research, reports, and scholarly works from the vibrant academic community at Kampala International University.
No matching results? Clear all filters to begin a fresh search.
Phenotypic and Molecular Characterization of Escherichia coli Isolates from Japanese quails (Coturnixcoturnix japonica) in Kelantan, Malaysia
Author: Samuel Mailafia, Desalini Nagappan; and Pwaveno Huladeino Bamaiyi
Publisher: ISOI Journal Of Microbiology, Biotechnology And Food Science
Published: 2017
Section: School of Allied Health Sciences
Abstract
This study was conducted to characterize E. coli isolates in Coturnixcoturnix japonica (Japanese quails). A total sample of 80 cloacal swabs was collected (40 from 2 months old quails and 40 from 3 weeks old quails). The isolates were confirmed using culture, microscopy and biochemical identification. Molecular typing using Polymerase chain Reaction(PCR) assay confirmed that 60 (75%) of the isolates to be E. coli and none of the isolates confirmed belonged E. coli 0157 serotype. Statistical analysis using Chi square showed no significant difference in prevalence of E.coli between the 2 months old and the 3 weeks old quails (P>0.05). Antibiotic susceptibility testing showed that there was high resistance of 75% of the E. coli isolates towards multiple antibiotics such as tetracycline and ampicillin while sensitivity of 75% was found for ciprofloxacin and gentamycin. Our findings suggests that E. coli infection may be endemic in the study area but serotype E. coli 0157 may be absent. The multiple drug resistances dissipated in our study calls for concern in the face of increasing limited antibiotic options and more searh for genuine antibiotics in managing colibacillosis on Malaysian quails. Proper hygiene regime and biosecurity measures are necessary to minimize the risk of spread of infection to the human population.Â