Abstract

Purpose: The use of medicine plants in the management of various human ailments have gained lots of attention in recent time; 
hence, the present study aimed to investigate the impact of Bidens pilosa on the behavioral, oxidative stress and cerebellar cortical 
histoarchitectural alterations during bisphenol A exposure in mice.
Methods: Thirty (30) adult male mice were divided into six groups. Group 1 received distilled water (2 mls/kg b.w). and group 2 was 
treated with (bisphenol A) BPA (100 mg/kg body weight). Groups 3–5 were co-treated with varying doses of Bidens pilosa (250 mg/ 
kg, 500 mg/kg, and 1000 mg/kg b.w). and group 6 received vitamin C (60 mg/kg b.w). along with BPA. The animals underwent 
neurobehavioral tests (beam walking and wire hang). Other parameters evaluated included body weight, oxidative stress biomarkers, 
and cerebellar histology.
Results: Animals treated with high doses of Bidens pilosa spent less time crossing the beam during the beam walking test and more 
time in the wire hang test than those treated with BPA alone. Lowered malondialdehyde level and higher catalase and superoxide 
dismutase activity was observed in Bidens pilosa treated groups than in the BPA-only group. Histological examination revealed 
a significant improvement in the cerebellar tissue structure in Bidens pilosa treated groups, particularly at higher doses.
Conclusion: Bidens pilosa demonstrated potential protective effects against BPA-induced oxidative stress and negative histological 
changes in the cerebellar cortex, suggesting its therapeutic potential for mitigating BPA neurotoxicity. Further research is needed to 
explore the therapeutic applicability.