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A method for the analysis of sugars in biological systems using reductive amination in combination with hydrophilic interaction chromatography and high resolution mass spectrometry
Author: Author links open oveSamiBawazeeraAliMuhsen AliacAliyahAlhawitiaAbedawnKhalafbColinGibsonbJonansTusiimireaDavid G.Watsona
Publisher: WestCHEM Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, UK
Published: 2017
Section: School of Pharmacy
Abstract
Separation of sugar isomers
extracted from biological samples is challenging because of their natural
occurrence as alpha and beta anomers and, in the case of hexoses, in their
pyranose and furanose forms. A reductive amination method was developed for the
tagging of sugars with the aim of it becoming part of a metabolomics work flow.
The best separation of the common hexoses (glucose, fructose, mannose and
galactose) was achieved when 2H5-aniline was used as
the tagging reagent in combination with separation on a ZICHILIC column. The
method was used to tag a range of sugars including pentoses and uronic acids.
The method was simple to perform and was able to improve both the separation of
sugars and their response to electrospray ionisation. The method was applied to
the profiling of sugars in urine where a number of hexose and pentose isomers
could be observed. It was also applied to the quantification of sugars in
post-mortem brain samples from three control samples and three samples from
individuals who had suffered from bipolar disorder.